Real-time measurements of protein dynamics using fluorescence activation-coupled protein labeling method

We present a fluorescence activation-coupled protein labeling (FAPL) method, which employs small-molecular probes that exhibit almost no basal fluorescence but acquire strong fluorescence upon covalent binding to tag-proteins. This method enables real-time imaging of protein labeling without any washout process and is uniquely suitable for real-time imaging of protein dynamics on the cell surface. We applied this method to address the spatiotemporal dynamics of the EGF receptor during cell migration.     

用"点击化学"法合成新型他克林-四氢异喹啉异二联体

从2-氯乙胺盐酸盐出发,经叠氮化与缩合反应制得9-(叠氮基乙基氨基)-1,2,3,4-四氢吖啶类衍生物(4a～4c);以6-庚炔-1-醇为原料,利用微波反应合成了2-(6.庚炔基)-6,7-二甲氧基-1-苯基-1,2,3,4-四氢异喹啉(7);4与7通过"点击化学"法合成了三个乙酰胆碱酯酶抑制剂-3个他克林-四氢异喹啉...     

1,5]-H shift of aldehyde hydrogen in dienal compounds to produce ketenes

In 3-ethoxycarbonyl-2,4-dienal compounds, a thermal [1,5]-H shift of aldehyde hydrogen easily proceeded to produce the corresponding vinyl ketenes due to the remarkable substituent effect caused by the C3 ester group. The produced ketenes were captured by an alcohol and olefins to afford the corresponding esters and four-membered ring compounds, respectively.     

Ultrafast spectroscopy and coherent acoustic phonons of Au-Ag core-shell nanorods

We performed the first investigations of coherent acoustic phonons in Au-Ag core-shell nanorods, which were compared with the results of parental Au nanorods. Both breathing and extensional modes were observed in Au-Ag core-shell nanorods with ~11 nm Ag shell while only extensional modes were detected in other core-shell nanorods with 4-7 nm Ag shell. Young's modulus estimated from the oscillation period of extensional modes was found to be larger for Au-Ag core-shell nanorods with ~4 nm Ag shell, as compared with that of Au nanorods. The value of Young's modulus decreases with the increase of the Ag shell thickness and finally becomes smaller than that of Au nanorods. This phenomenon is interpreted in terms of the surface effects and the existence of grain boundaries in the lattice structure of Ag shell.     

ITER CH HCSB TBM一维中子学优化设计

1引言 ITER实验包层模块（Test Blanket Modules，TBM）是验证未来聚变反应堆能否实现氚自持、高热量提取的重要实验平台，也是将来发展DEMO聚变堆包层技术而进行电磁性能测试、热工水力学测试、氚增殖实验的重要工具。由于ITER对自身安全的严格要求和对TBM安全性的要求限制，TBM内部能量产生的多少和最大功率密度及其分布等参数都非常重要。同时，产氚实验也是TBM重要的目的之一，它关系到热核聚变堆氚燃料的供给。因此，提高TBM的氚增殖性能的优化设计同样具有十分重要的意义。     

3keV氘离子在氚增殖材料Li2TiO3中的理化行为研究

在ITER固态实验包层设计中，固态氚增殖材料的性能是决定包层产氚能力的重要因素。钛酸锂（Li2TiO3）是非常重要的一种固态增殖材料，为了理解氢的同位素在Li2TiO3中的理化行为，本工作采用XPS和TDS实验设备开展了用3keV D2^＋离子源注入Li2TiO3材料的研究.通过实验，发现了4种氘的热解析峰，它们分别来源于表面吸附的氘离子，表面吸附的氘离子形成的D2O，材料体内E’-center俘获的氘离子和材料体内以O—D化学键态形式存在的氘离子。由于中子辐照损伤更容易在材料体内形成，E’-center俘获的氘离子和O—D键这两种化学态对我们理解氚的化学行为更加重要。Ti-2p电子态的X射线光电谱表明，随着氘离子通量增加，Ti^4＋转换成了Ti^3＋。从O-1s电子态谱看出，随着氘离子通量增加，有一个峰消失了。关于这个现象，还需做更进一步的实验研究。     

丝阵Z箍缩等离子体内爆过程单丝特性研究

研究了丝阵Z箍缩等离子体内爆过程中单丝行为、物理参数匹配、X射线发射情况。根据丝阵Z箍缩等离子体动力学一维模型研制了相关的计算程序，对单丝的内爆过程进行了数值模拟，对丝阵箍缩匹配关系进行了计算，给出了丝阵初始半径、电流脉冲宽度和峰值电流的匹配关系，同时对丝阵内的最小丝间距、材料的选择等问题进行优化研究。     

“氚坑深度”和“氚坑时间”

裂变堆中的氙-135中毒效应是由于裂变产生的碘-135经由β衰变产生氙。135，后者吸收中子的截面很大，如停堆时剩余反应性不够，就要经过一段“碘坑时间”才能恢复到原来停堆前的反应性后才正常工作起来。与此类似却有所不同的一个全新的概念，“氚坑深度”和“氚坑时间”首次被我们引入到聚变堆研究领域，它表明为了实现“得失相当”，起动一个聚变堆所要求的最少氚储备和运行时间。“氚坑深度”和“氚坑时间”与具体的氚回收方案、提取氚的工艺过程、堆部件材料中的氚扣留量、增殖剂中不可回收的氚份额、泄漏到堆大厅的包容惰性气体氦中的份额以及氚自然衰变等等有关。     

球形环嬗变堆包层冷却管道热工设计与热结构力学分析

利用球形环托卡马克作为聚变中子源、处置裂变堆长寿命放射性核废料的嬗变堆作为聚变能早期应用的重要途径。因核废物的日益积累和已具备现实的技术基础而受到广泛的重视。也是目前国际聚变界研究的前沿课题。     

Suppression of MeIQ-induced SOS response by allylbenzenes from Asiasarum heterotropoides in the Salmonella typhimurium OY1001/1A2 umu test

Three allylbenzenes from Asiasarum heterotropoides, methyleugenol (1), elemicin (2) and gamma-asaron (3) showed suppressive effects on umu gene expression of the SOS response in the Salmonella typhimurium OY1001/1A2 umu test against the mutagen 2-amino-3,4-dimethylimidazo[4,5-f ]quinoline (MeIQ). Gene expression was suppressed 70.0, 75.9 and 81.7% at a concentration of 0.4 mM, respectively. The ID50 values (50% inhibition dose) of these compounds were 0.125, 0.098 and 0.059 mM, respectively. On the other hand, compounds 1-3 showed weak suppressive effects of the SOS-inducing activity on activated MeIQ.